Titre du document / Document title

Adhesion and colonization of Vibrio cholerae O1 on shrimp and crab carapaces = Adhérence et colonisation de Vibrio cholerae O1 sur des carapaces de crevettes et de crabes

Auteur(s) / Author(s)

CASTRO-ROSAS J. ⁽¹⁾ ; ESCARTIN E. F. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Facultad de Química, Universidad Autónoma de Querétaro, Querétaro 76010, MEXIQUE

Résumé / Abstract

The potential of Vibrio cholerae O1 to attach to and colonize the carapaces of shrimp and crabs was evaluated. One million cells of V. cholerae O1 were spread within a circle on the external surfaces of separated carapaces and stored at 22 ± 0.2°C in a moist environment to permit adherence. Attached vibrios were counted directly by an immunofluorescence technique and by the pour plate technique after detachment of the cells. To study the colonization process, rifampicin-resistant strains of V. cholerae O1 were used. V. cholerae Ol strains, including those resistant to rifampicin, were able to attach to shrimp and crab carapaces. Dorsal crab carapaces showed higher levels of attachment than ventral carapaces. Colonization of V. cholerae O1 on these carapaces was also demonstrated. Both attachment and colonization on the shrimp exoskeleton were optimal at a salinity of 1.0 to 1.5%, a pH of 6.0 to 7.0, and a temperature of 37°C. Less than 2% attachment at 3°C contrasted with >20% attachment at 37°C. Even at 3% NaCI, some attachment was observed. Although attachment percentages may appear low (2 to 20%), they represent significant numbers, about 3.7 to 5.6 log₁₀ CFU per carapace. A rugose V. cholerae O1 strain attached to and colonized the shrimp carapace in a fashion very similar to that of the smooth strain from which it was derived. The ability of V. cholerae O1 to attach to and colonize exoskeletons of edible crustaceans provides a potential means of survival in aquatic environments. Concentrations of vibrios that may be reached on a single crab or shrimp carapace are clearly of concern with regard to public health.

Cette étude évalue la capacité de Vibrio cholerae O1 à s'attacher et se multiplier sur les carapaces de crevettes et de crabes. Les carapaces dorsales de crabes montrent plus d'adhérabilité que les carapaces ventrales. La capacité de Vibrio cholerae O1 à adhérer et coloniser les carapaces de crustacés leur fournit un moyen de survie dans les environnements aquatiques. Elle varie en fonction des conditions du milieu comme la salinité, le pH et la température. Les concentrations détectées sur ces carapaces sont à considérer avec attention.

Revue / Journal Title

Journal of food protection   ISSN 0362-028X   CODEN JFPRDR 

Source / Source

2002, vol. 65, n^o3, pp. 492-498 (35 ref.)

Langue / Language

Anglais

Editeur / Publisher

International Association of Milk, Food and Environmental Sanitarians, Des Moines, IA, ETATS-UNIS  (1977) (Revue)

Mots-clés anglais / English Keywords

Environmental factor ; Edible crustacean ; Brachyura ; Invertebrata ; Arthropoda ; Crustacea ; Decapoda ; Macrura ; Bacteria ; Vibrionaceae ; Sodium Chlorides ; Temperature ; pH ; Crab ; Prawn ; Vibrio cholerae ; Carapace ; Microorganism growth ; Adhesion ; Biological contamination ;

Mots-clés français / French Keywords

Facteur milieu ; Crustacé comestible ; Brachyura ; Invertebrata ; Arthropoda ; Crustacea ; Decapoda ; Macrura ; Bactérie ; Vibrionaceae ; Sodium Chlorure ; Température ; pH ; Crabe ; Crevette ; Vibrio cholerae ; Carapace ; Multiplication microorganisme ; Adhérence ; Contamination biologique ;

Mots-clés espagnols / Spanish Keywords

Factor medio ; Crustáceo comestible ; Brachyura ; Invertebrata ; Arthropoda ; Crustacea ; Decapoda ; Macrura ; Bacteria ; Vibrionaceae ; Sodio Cloruro ; Temperatura ; pH ; Cangrejo de mar ; Camarón ; Vibrio cholerae ; Exoesqueleto ; Multiplicación microorganismo ; Adherencia ; Contaminación biológica ;

Localisation / Location

INIST-CNRS, Cote INIST : 547, 35400010038751.0080